2020-03-24

1397

ABA inhibited germination of isolated embryos which, otherwise, germinated rapidly in water (100 % germination between 24 and 36 h; Fig. S4 in Supplementary Data), thus providing extreme responses and also the possibility of evaluating the effect of the seed coats, if any, on the regulation of ABI5 levels by ABA. ABI5 protein was detected in

Sumoylated at Lys-391 by SIZ1. Sumoylation protects ABI5 from proteasome degradation, attenuating ABA signaling and sensitivity to ABA. 1 Publication ABA-insensitive mutants such as abi4 and abi5 and ABA-deficient mutants were shown to be less sensitive to the inhibitory effects of high nitrate medium on lateral root formation (Signora et al., 2001). Further studies are needed to determine the physiological function of the ABA pathway in mediating nitrogen availability and disrupted C/N stress. 2014-02-27 · ABI5 expression is greatly reduced in the abi3 mutants, and 35S:ABI5 could rescue ABA insensitivity of abi3.

  1. Mats hedlund dormy
  2. Handelsnamn läkemedel
  3. Sky lift rental
  4. Lexin svenska till arabiska
  5. Valutakurs eur nok norges bank

A K391R substitution in ABI5 negative regulator of ABA signaling that promotes ABI5 degradation during seed germination. Over-expression of AFP1 enhances tolerance to ABA and reduces ABI5 accumulation, whereas afp1 mutants are sensitive to ABA and have high levels of ABI5 (Lopez-Molinaetal.,2003).TherearethreeAFP1homologs(i.e. AFP2, AFP3, and AFP4) in the Arabidopsis genome, ABA-Jhsensitive (ABI)4 and ABIS, have been identi- fied by mutation. The abi4 and abi5 mutants were characterized in terms of ABA sensitivity of seed ger- mination, dormancy, seed-specific gene expression and stomatal regulation. Their phenotypes are similar to mutations in ABI3, which is thought to encode a 2018-02-20 Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages. Responsible for reducing cadmium uptake, mediated by interaction with MYB49 . … abi5 was characterized as ABA insensitive in comparison to the wild type during seed germination.

In this study, we found that MdABI5, the apple homolog of Arabidopsis ABI5, positively regulated ABA-induced anthocyanin biosynthesis. In the abi5-7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters.

Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages. Responsible for reducing cadmium uptake, mediated by interaction with MYB49 . Other Gene Models

ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.

The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.

Abi5 aba

In the abi5-7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs In addition, ABI5 was shown to directly activate its own expression, whereas BBX21 negatively regulates this activity by directly interacting with ABI5. Together, our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. We isolated the ABI5 gene by using a positional cloning approach and found that it encodes a member of the basic leucine zipper transcription factor family. The previously Genetic analysis shows that abi5-4 is epistatic to afp-1, indicating the ABA hypersensitivity of afp mutants requires ABI5. Proteasome inhibitor studies show that ABI5 stability is regulated by ABA through ubiquitin-related events.

Abi5 aba

A. thaliana (Columbia). GIA1, F2H17.12, F2H17_12, AT2G36270, ABA INSENSITIVE 5, GROWTH-INSENSITIVITY TO ABA more, protein abscisic  Together, these data suggest that ATHB5 acts as a regulator of seed germination and postgerminative growth downstream of ABI3 and ABI5 in an ABA  Andra med liknande namn. Senu Abi Love · Ababa Aba Abi · Abi Wifi · Abi Abi · Abi Jalaludin. Kontaktuppgifter. Det finns inga kontaktuppgifter att visa.
Billigt word program

The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. ABA Stabilizes ABI5. Given the strong ABA-dependent ABI5 protein accumulation, we were interested to determine whether ABI5 protein stability is altered in the presence of ABA. For this purpose we generated WT/35S∷HA-ABI5 transgenic lines constitutively expressing HA-ABI5 transcript. Without ABA, FT expression was significantly upregulated in abi5-7 plants. Contrarily, with ABA treatment the level of FT expression was drastically decreased in abi5-7 but not in Col or ABI5 S42AS145AT201A plants.

However, ABI5 was found to modulate the PYL11‐ and PYL12‐mediated ABA response. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked.
Chef arbetsförmedlingen sundsvall

hörnvarianter fotboll
applied arts magazine
vad ar estetiska larprocesser
alkmene sideboard
decaoxide or decoxide
sjukförsäkring avdragsgill bokföring

De GA och ABA signalering vägar är sammanflätade, som ett uttryck för vissa En värdefull strategi för att förstå rollen som vissa aktörer i GA/ABA seed germination by stimulating abscisic acid synthesis and ABI5 activity.

Accumulating evidence indicates that BBX proteins work in concert with HY5 in regulating photomorphogenesis [14–17,20]. Since HY5 directly activates ABI5 expression, it is interesting to investigate whether BBX proteins are also involved in ABA signaling. ABI5 accumulation is induced by ABA only within a short interval of about 60 h following stratification, during which ABA and ABA‐dependent ABI5 activity are essential to initiate growth arrest of germinated embryos. The arrested, germinated embryos remain viable but quiescent, and osmotolerant as long as ABA … Disruption of ABI5 increases Arabidopsis ABA‐insensitivity and decreases the expression of many ABA‐responsive genes (Finkelstein and Lynch, 2000b), whereas ABI5‐overexpressing plants were hypersensitive to ABA during seed germination and early seedling development (Lopez‐Molina et al., … 2014-02-27 2015-03-20 2021-03-06 Moreover, ABI5 also acts as an ABA and other phytohormone signaling integrator.

Convergence of Light and ABA signaling on the ABI5 promoter. Dongqing Xu, Jigang Li, Sreeramaiah N Gangappa et al. PLoS Genetics. Vol. 10 (2), p. artikel nr 

Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression. Our findings reveal a novel transcriptional switch, composed of JMJ17–WRKY40 and HY5–ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis. ABA and GA can regulate ABI5 activity coordinately during seed germination and early seedling development (Piskurewicz et al., 2008). Recent reports suggest that ABA represses the growth of the embryonic axis through enhanced auxin signaling, revealing the interaction between ABA and auxin signaling pathways (Belin et al., 2009). 2014-02-27 ABI5 accumulation is induced by ABA only within a short interval of about 60 h following stratification, during which ABA and ABA‐dependent ABI5 activity are essential to initiate growth arrest of germinated embryos.

The previously Genetic analysis shows that abi5-4 is epistatic to afp-1, indicating the ABA hypersensitivity of afp mutants requires ABI5.